cell basal media  (ATCC)

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: Citrullinated and Homocitrullinated Proteins/Peptides are Present in DBA/1J Metatarsophalangeal Joints. A) Representative hematoxylin + eosin (H&E) at 80× magnification and immunofluorescent micrographs at 20× magnification of joints from DBA/1J mice immunized with bovine type II collagen (arthritic: N = 9; non-arthritic: N = 10) or PBS (N = 9). Scale bars represent 500 μm. Max projections of Z-stacks show nuclei (DAPI; blue), citrullinated proteins/peptides (CitP; green), and homocitrullinated proteins/peptides (HomoCitP; red). Corrected total fluorescence intensity for B) CitP and C) HomoCitP in three joint structures: bone marrow, synovium, and cartilage. Graphs show the median [IQR], with each symbol representing an individual mouse. Statistical analysis was performed using Kruskal-Wallis with Dunn's multiple comparisons test, and the resulting p-values were p = 0.0046, p = 0.0084, p = 0.4076, p = 0.0143, p = 0.0136, and p = 0.1733 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

Article Snippet: Following application of TrueView autofluorescence quencher, slides were mounted using Vectashield Vibrance Antifade mounting media with DAPI (SP-8500-15; Vector Laboratories; USA) and air dried for 2 h before imaging on the Nikon Ti2-E microscope.

Techniques: Fluorescence

Journal: Molecular Microbiology

Article Title: Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose Epimerase

doi: 10.1111/mmi.70043

Figure Lengend Snippet: Growth analyses of C. japonicus strains grown in MOPS defined media supplemented with 0.5% (w/v) of (A) mannobiose, (B) mannotriose, (C) mannosyl‐glucose, (D) cellobiose, (E) glucomannan, (F) carob galactomannan, (G) guar galactomannan, or (H) linear mannan. Notably, panels A—D were grown on a separate microplate from panels E—F and are shown together for ease of visualization. Growth analyses were conducted using an EPOCH2 microplate reader (Biotek) in biological triplicate for 48 h. Error bars depict standard deviation but are too small to be observed in some graphs. Complete growth statistics are available in Table .

Article Snippet: C. japonicus strains were cultured in MOPS minimal medium from TekNova (cat. no. M2106), supplemented with 1.32 mM phosphate and 0.2% (w/v) glucose.

Techniques: Standard Deviation

Journal: Molecular Microbiology

Article Title: Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose Epimerase

doi: 10.1111/mmi.70043

Figure Lengend Snippet: Growth analyses of C. japonicus single gene deletions, intermediate strains, and double ectopic complementation strains grown in MOPS defined media supplemented with 0.5% (w/v) of either (A) mannobiose, (B) mannotriose, (C) glucomannan, (D) carob galactomannan, (E) guar galactomannan, or (F) linear mannan. Panels A, B, and F were grown on a separate microplate from Panels C, D, and E, and are shown here together for ease of visualization. Growth analyses were conducted using an EPOCH2 microplate reader (Biotek) in biological triplicate for 48 h. Error bars depict standard deviation but are too small to be observed in many of the graphs. Complete growth statistics are available in Table .

Article Snippet: C. japonicus strains were cultured in MOPS minimal medium from TekNova (cat. no. M2106), supplemented with 1.32 mM phosphate and 0.2% (w/v) glucose.

Techniques: Standard Deviation

Journal: Molecular Microbiology

Article Title: Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose Epimerase

doi: 10.1111/mmi.70043

Figure Lengend Snippet: Growth analyses of C. japonicus pJKN5 complement strains grown in MOPS defined media supplemented with 0.5% (w/v) of either (A) mannobiose, (B) mannotriose, (C) glucomannan, (D) carob galactomannan, (E) guar galactomannan, or (F) linear mannan. Notably, panels A, B, and F were grown on a separate microplate from panels C, D, and E and are shown together for ease of visualization. Growth analyses were conducted in an EPOCH2 microplate reader (Biotek) in biological triplicate for 48 h. Error bars depict standard deviation and complete growth statistics are available in Table .

Article Snippet: C. japonicus strains were cultured in MOPS minimal medium from TekNova (cat. no. M2106), supplemented with 1.32 mM phosphate and 0.2% (w/v) glucose.

Techniques: Standard Deviation